Blar i forfatter "Jakubec, Martin"

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    • Bacterial extracellular vesicles: towards realistic models for bacterial membranes in molecular interaction studies by surface plasmon resonance 

      Brilkov, Maxim; Stenbakk, Victoria; Jakubec, Martin; Vasskog, Terje; Kristoffersen, Tone; Cavanagh, Jorunn Pauline; Ericson, Johanna Ulrica; Isaksson, Johan Mattias; Flaten, Gøril Eide (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-13)
      One way to mitigate the ongoing antimicrobial resistance crisis is to discover and develop new classes of antibiotics. As all antibiotics at some point need to either cross or just interact with the bacterial membrane, there is a need for representative models of bacterial membranes and efficient methods to characterize the interactions with novel molecules -both to generate new knowledge and to ...

    • Contrasting the phospholipid profiles of two neoplastic cell lines reveal a high PC:PE ratio for SH-SY5Y cells relative to A431 cells 

      Bariås, Espen; Jakubec, Martin; Førsund, Elise; Hjørnevik, Linda Veka; Lewis, Aurelia Eva; Halskau, Øyvind (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-03-14)
      Lipids have been implicated in Parkinson's Disease (PD). We therefore studied the lipid profile of the neuroblastoma SH-SY5Y cell line, which is used extensively in PD research and compared it to that of the A431 epithelial cancer cell line. We have isolated whole cell extracts (WC) and plasma membrane (PM) fractions of both cell lines. The isolates were analyzed with 31P NMR. We observed a significant ...

    • Goldilocks Dilemma: LPS Works Both as the Initial Target and a Barrier for the Antimicrobial Action of Cationic AMPs on E. coli 

      Jakubec, Martin; Rylandsholm, Fredrik G.; Rainsford, Philip; Silk, Mitchell; Bril'kov, Maxim; Kristoffersen, Tone; Juskewitz, Eric; Ericson, Johanna U; Svendsen, John Sigurd Mjøen (Journal article; Tidsskriftartikkel, 2023-07-20)
      Antimicrobial peptides (AMPs) are generally membrane-active compounds that physically disrupt bacterial membranes. Despite extensive research, the precise mode of action of AMPs is still a topic of great debate. This work demonstrates that the initial interaction between the Gram-negative E. coli and AMPs is driven by lipopolysaccharides (LPS) that act as kinetic barriers for the binding of AMPs to ...

    • Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis 

      Rainsford, Philip; Rylandsholm, Fredrik G.; Jakubec, Martin; Silk, Mitchell; Juskewitz, Eric; Ericson, Johanna U; Svendsen, John Sigurd Mjøen; Engh, Richard Alan; Isaksson, Johan Mattias (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-03)
      One strategy to combat antimicrobial resistance is the discovery of new classes of antibiotics. Most antibiotics will at some point interact with the bacterial membrane to either interfere with its integrity or to cross it. Reliable and efficient tools for determining the dissociation constant for membrane binding (K<sub>D</sub>) and the partitioning coefficient between the aqueous- and membrane ...

    • Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis 

      Rainsford, Philip Ben; Rylandsholm, Fredrik G.; Jakubec, Martin; Silk, Mitchell; Juskewitz, Eric; Ericson, Johanna Ulrica; Svendsen, John Sigurd Mjøen; Engh, Richard Alan; Isaksson, Johan Mattias (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-03)
      One strategy to combat antimicrobial resistance is the discovery of new classes of antibiotics. Most antibiotics will at some point interact with the bacterial membrane to either interfere with its integrity or to cross it. Reliable and efficient tools for determining the dissociation constant for membrane binding (K<sub><i>D</sub></i>) and the partitioning coefficient between the aqueous- and ...

    • Lulworthinone: In Vitro Mode of Action Investigation of an Antibacterial Dimeric Naphthopyrone Isolated from a Marine Fungus 

      Juskewitz, Eric; Mishchenko, Ekaterina; Dubey, Vishesh Kumar; Jenssen, Marte; Jakubec, Martin; Rainsford, Philip; Isaksson, Johan Mattias; Andersen, Jeanette Hammer; Ericson, Johanna U (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-04-21)
      Treatment options for infections caused by antimicrobial-resistant bacteria are rendered ineffective, and drug alternatives are needed—either from new chemical classes or drugs with new modes of action. Historically, natural products have been important contributors to drug discovery. In a recent study, the dimeric naphthopyrone lulworthinone produced by an obligate marine fungus in the family ...

    • WIND-PVPA: Water/Ion NMR Detected PVPA to assess lipid barrier integrity in vitro through quantification of passive water- and ion transport 

      Rainsford, Philip Ben; Sarre, Birta R.; Falavigna, Margherita; Brandsdal, Bjørn Olav; Flaten, Gøril Eide; Jakubec, Martin; Isaksson, Johan Mattias (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-22)
      Water/Ion NMR Detected – Phospholipid Vesicle Permeability Assay (WIND-PVPA), is presented as a novel, straightforward and automatable method to assess lipid barrier integrity in vitro. The apparent permeability constants of water- and ions across the PVPA barriers are determined in a one-pot experiment under the influence of membrane-active guest molecules. NMR spectroscopy is used to quantify ...